Clostridium sporogenes spore suspension|BNCC362134 |BNCC

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Clostridium sporogenes spore suspension

Subculture	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculturing	This spore suspension is a pure spore suspension, which is obtained by cell separation and collection of spores. It can be used directly, and the corresponding volume can be added according to the test requirements.
Growth conditions	37 ℃,5-7 days, anaerobic
Storage conditions	2-8 ℃
Safety level	2
Morphology	Liquid medium turbid, G + (blue-purple), cocci, purity: pure
Application	Research; Analysis and testing. Teaching and research. "Technical Specifications for Disinfection" Bacteria for Testing Sterilization Effect of Traditional Chinese Medicine Therapeutics, "GB/T 4789.12-2003 Clostridium botulinum and Botulinum Toxin Test" Positive Control Strain, Quality Control Strain of Chinese Pharmacopoeia, 2015 Edition. 2020 Edition Pharmacopoeia-"General Principles 1100 Biological Inspection Method" for tested strains of bacteria in pharmacopoeia, medium "sensitivity inspection" for tested strains, "method applicability test" for tested strains, "medium applicability test and control bacteria test method applicability test" for tested strains
Sharing mode	Public welfare sharing