Cupriavidus necator DNA standard|BNCC363254 |BNCC

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Cupriavidus necator DNA standard

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	When this standard sample is used, it is moved to room temperature (20~25 ℃) for re-melting, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, so as to avoid repeated freezing and thawing.
Growth conditions	30 ℃;18-24 hours; Aerobic
Storage conditions	-80 ℃
Safety level	1
Application	This hookworm copper-greedy DNA standard, through fluorescence quantitative pcr combined with digital PCR fixed value, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing