Weiyel Reference Materials
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| Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
| Subculturing | Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μl. |
| Growth conditions | 37 ℃;24-48h;5% CO2 + 95% air |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Isolation | Cervix, Illinois |
| Application | The liquid indoor quality control product is Neisseria gonorrhoeae inactivated bacteria suspension, through fluorescence quantitative pcr combined with digital PCR fixed value, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. |
| Sharing mode | Public welfare sharing |
Aspergillus fumigatus DNA standard
BNCC363587
Candida krusei nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC364638
Monkeypox Virus B6R Pseudovirus standard quality control product
BNCC362591
Bacteroides fragilis nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC371828
Human herpesvirus 3 DNA standard(varicella-zoster virus)
BNCC363066
Salmonella enterica nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC361552