Weiyel Reference Materials
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| Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
| Subculturing | Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μl. |
| Growth conditions | 37 ℃;24-48h;5% CO2 + 95% air |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Isolation | Cervix, Illinois |
| Application | The liquid indoor quality control product is Neisseria gonorrhoeae inactivated bacteria suspension, through fluorescence quantitative pcr combined with digital PCR fixed value, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. |
| Sharing mode | Public welfare sharing |
Escherichia bacteriophage MS2 nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC362700
Corynebacterium ulcerans nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC361625
Aspergillus fumigatus DNA lab quality control
BNCC363592
Human herpesvirus 5 DNA standard
BNCC363368
Goat Pox virus DNA standard
BNCC363209
Escherichia phage T7 nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC362908