Weiyel Reference Materials
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| Subculture | Special peptone: 23.0, soluble starch: 1.0, sodium chloride: 5.0, agar: 10.0,pH:7.3±0.2(25 ℃) |
| Subculturing | ① Prepare 1-2 pieces of the above-mentioned plates; ② After disinfection of the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL sterile water to freeze the tube, fully dissolve the bacterial powder, and then put it into the plate at 200 μL/piece, and coat it evenly. ④ Place the plate under the above culture conditions, and the strain can be used. |
| Growth conditions | 37 ℃;24-48h;5% CO2 |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| Morphology | The colony diameter is 1-2mm, round, neat edges, opaque, gray-white front, raised in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), Vibrio |
| Isolation | The feces of the marmoset |
| Application | "GB4789.28 quality requirements for media and reagents" standard strains. Quality control strains, intestinal and emerging infectious disease research. |
| Sharing mode | Public welfare sharing |
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