Weiyel Reference Materials
Subculturing | Sample processing: sample processing is carried out according to GB 4789.30-2016 National Food Safety Standard for Listeria monocytogenes Detection; Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15 μL, template 1 μL, sterile ddH2O 9 μL Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 57 ℃, 30s, 40cycle; Fluorescence channel: FAM; |
Safety level | 0 |
Application | It is suitable for auxiliary diagnosis of Listeria monocytogenes infection in water samples, suspected contaminated water, food and other samples. |
Sharing mode | Public welfare sharing |