Pseudomonas aeruginosa nucleic acid detection kit (QPCR)|BNCC361453 |BNCC

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Pseudomonas aeruginosa nucleic acid detection kit (QPCR)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Experimental preparation: Before the experiment, the sample was cultured with reference to T/SDAQI 005-2021; the various components in the kit were dissolved on ice, slightly mixed and temporarily centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 18.5 μL, template 1 μL, sterile ddH2O5.5μL Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 50 ℃, 30s, 40cycle; Fluorescence channel: FAM.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-20 ℃, cold storage
Safety level	2
Isolation	Blood culture
Application	It is used for rapid detection of Pseudomonas aeruginosa in food and cosmetics.
Sharing mode	Public welfare sharing