Biochemical ldentification Kit for Streptococcus thermophilus|BNCC361982 |BNCC

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Biochemical ldentification Kit for Streptococcus thermophilus

Subculture	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculturing	Streptococcus thermophilus was cultured at 37 ℃, 5% CO2 and 48h on Columbia blood plate. A thick layer of bacteria mud was grown on the plate, and the eluted bacteria liquid was used as standard inoculation solution to test the reagent. The standard inoculation solution was inoculated into inulin, lactose, mannitol, salicylate, sorbitol, aescin and hippuric acid reagent respectively according to requirements, and put into 37 ℃ and 5% CO2 incubator after inoculation.
Growth conditions	Culture temperature 37 ℃;24-48 hours; Gas environment 5% CO2;
Storage conditions	-20 ℃
Safety level	0
Application	It is suitable for physiological and biochemical identification of Streptococcus thermophilus in GB4789.35-2016 "Food Microbiological Examination of Lactic Acid Bacteria"
Sharing mode	Public welfare sharing