Ectromelia virus nucleic acid detection kit (QPCR)|BNCC363563 |BNCC

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Ectromelia virus nucleic acid detection kit (QPCR)

Subculturing	Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template (1-10)μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 54 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence channel: FAM.
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	It is suitable for in vitro amplification research and detection of murine pox virus, and cannot be used for clinical diagnosis of diseases.
Sharing mode	Public welfare sharing