Rhodopseudomonas palustris DNA lab quality control|BNCC371979 |BNCC

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Rhodopseudomonas palustris DNA lab quality control

Subculture	Ammonium chloride: 1.0, potassium dihydrogen phosphate: 0.5, magnesium chloride: 0.5, calcium chloride: 0.1, sodium chloride: 1.0, sodium acetate: 2.0, sodium succinate: 1.0, yeast extract powder: 0.5, peptone: 0.5, ferrous chloride: 1.8mg, cobalt chloride: 0.25mg, nickel chloride: 0.01mg, copper chloride: 0.01mg, manganese chloride: 0.07mg, zinc chloride: 0.1mg, boric acid: 0.5mg, sodium selenite: 0.01mg, sodium molybdate: sodium molybdate: 0.01g, biotin: 0.01mg, nicotinic acid: 0.035mg, calcium pantothenate: 0.01mg, thiamine hydrochloride: 0.03mg,VB12:0.005mg, pyridoxamine hydrochloride: 0.01mg, p-aminobenzoic acid: 0.02mg, agar: 13.0,pH:6.8(25 ℃)
Subculturing	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	30 ℃,3-4 days, anaerobic light
Storage conditions	-80 ℃
Safety level	0
Application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of red Pseudomonas palustris, and can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. through digital PCR value.
Sharing mode	Public welfare sharing