Standard quality control sample of total coliforms in drinking water (Membrane membrane method)|BNCC360416 |BNCC

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Standard quality control sample of total coliforms in drinking water (Membrane membrane method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 200mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biosafety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of it is transferred to the above sterile bottle, which is a standard quality control sample with a total concentration of 200mL and a concentration of 10-100CFU/100mL.
Growth conditions	37°C, aerobic, 18-24h
Storage conditions	-20 ℃, kept away from light
Safety level	2
Application	Specific use: Quality control samples of total coliform bacteria in drinking water (filter membrane method) Applicable Standard: GB/T5750.12-2006 "Microbiological Indicators of Standard Test Methods for Drinking Water"
Sharing mode	Public welfare sharing