Seawater total coliform positive control quality control sample (Multi-tube fermentation method)|BNCC363180 |BNCC

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Seawater total coliform positive control quality control sample (Multi-tube fermentation method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	The sample requires a total of 150 mL of sterile water or sterile normal saline to dissolve. Before use, the quality control sample is placed at room temperature for 30 min, then opened in the biological safety cabinet, 3-4 mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of it is transferred to the above-mentioned sterile bottle, which is the quality control sample suspension with a total concentration of 150 mL and a concentration of 800-8000 MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-20 ℃
Safety level	0
Application	Applicable to the "GB 17378.7-2007 Marine Monitoring Specification Part 7: Ecological Investigation and Biological Monitoring of Offshore Pollution" standard, the detection of total coliforms in seawater, quality control and personnel assessment.
Sharing mode	Public welfare sharing