Standard quality control sample of coliform bacteria (plate counting method)|BNCC360592 |BNCC

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Standard quality control sample of coliform bacteria (plate counting method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 20min, then opened in a biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of it is transferred to the above sterile bottle, which is a standard quality control sample with a total concentration of 100mL and a concentration of 10 2~10 4 CFU/mL.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
Application	Quality control and personnel assessment of coliform count in food. Reference Standard "GB4789.3-2016 Food Safety National Standard Food Microbiological Detection Coliform Count" Plate Counting Method
Sharing mode	Public welfare sharing