Escherichia coli bacteriophage PhiX174 standard quality control product|BNCC362983 |BNCC

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Escherichia coli bacteriophage PhiX174 standard quality control product

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	When using the essential control product, it should be moved to room temperature (20~25 ℃) for 10 minutes, the concentration value is 10 9~10 10PFU/mL, and the reference concentration is 1.71 × 10 9PFU/mL
Safety level	1
Application	The Escherichia coli phage PhiX174 quantitative standard quality control products, and collected phages by culturing host bacteria of Escherichia coli and phage PhiX174. The phage titer and lysis efficiency were determined by double-layer agar counting method, which was mainly used for phage PhiX174 positive quality control.
Sharing mode	Public welfare sharing