Candida albicans DNA lab quality control|BNCC363222 |BNCC

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Candida albicans DNA lab quality control

Subculture	YM medium (English name: YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculturing	Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μl.
Growth conditions	30 ℃;24-48h; Aerobic;
Storage conditions	-80 ℃
Safety level	1
Isolation	Blood, Iowa
Application	The liquid indoor quality control is Candida albicans inactivated bacteria suspension, through the extraction of nucleic acid, the use of fluorescence quantitative pcr and digital PCR fixed value, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control.
Sharing mode	Public welfare sharing